Isolation of Beta-Lactamase Producing Genes (SHV, CTX-M1, CTX-M2, CTX-M3) in Escherichia coli Isolated from Pregnant Woman Patients

Authors

1 Zabol Medicinal Plants Research Center, Zabol University of Medical Sciences, Zabol, Iran

2 Department of Midwifery, Faculty of Nursing and Midwifery, Zabol University of Medical Sciences, Zabol, Iran

3 Maternal and Fetal Health Research Center, Department of Obstetrics and Gynecology, Zabol University of Medical Sciences, Zabol, Iran

Abstract

Background: In recent decades, extended spectrum beta-lactamase (ESBL) generating bacteria have increased universally. Among the most important causative agents of nosocomial infections throughout the world, Escherichia coli as main ESBL-producing bacteria are so highly regarded. Trends in the treatment of infections by such bacteria have led to a global concern.
 
Methods: All strains were cultured and identified by the Clinical Microbiology Laboratory and were recovered from blood and urine cultures. In-vitro presence of ESBL was confirmed with Clinical and Laboratory Standard Institute double disc and PCR for CTX-M1, CTM-M2, CTX-M3 method.
 
Results: The results of this study showed that Escherichia coli samples were resistant to AN (42.85%), GM (28.57%), AM (35.71%), AMC (35.71%), CZ (35.71%), and AZM (50%) antibiotics. While the most susceptible to antibiotic was ampicillin (64.28%), the least resistance to antibiotics was gentamicin.
 
Conclusion: The current situation of multiple bacterial antibiotic resistance has become a worrisome issue in UTI. Multi-drug-resistant E. coli can be readily encountered in hospital settings during daily clinical practice, and urologist should act timely. The management of such infections is extremely important for the future, with particular reference to prevention of new antibiotic resistance patterns.

Keywords


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Volume 1, Issue 1
July 2018
Pages 21-29
  • Receive Date: 13 October 2017
  • Revise Date: 25 February 2017
  • Accept Date: 19 May 2017
  • First Publish Date: 01 July 2018